pegfp n1 fus tls flagc Search Results


human fus  (Addgene inc)
93
Addgene inc human fus
Photoactivation of <t>FUS</t> condensation. (A) Scheme of full-length FUS protein (top) and chimeric constructs used in this study <t>(bottom)</t> <t>(IDR,</t> intrinsically disordered region; RRM and RGG, RNA-binding motives; Zn, Zn-finger). (B) Cartoon of the imaging assay in HEK cells. (C) Representative images (z-projections) before (left) and after (right) photoactivation of 1 min at 0.02 mW 488 nm-laser power at the output. Scale bar, 10 μm. (D) Line profiles of highlighted regions in (C) (dotted line, before activation; full line, after activation).
Human Fus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human fus/product/Addgene inc
Average 93 stars, based on 1 article reviews
human fus - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier

Image Search Results


Photoactivation of FUS condensation. (A) Scheme of full-length FUS protein (top) and chimeric constructs used in this study (bottom) (IDR, intrinsically disordered region; RRM and RGG, RNA-binding motives; Zn, Zn-finger). (B) Cartoon of the imaging assay in HEK cells. (C) Representative images (z-projections) before (left) and after (right) photoactivation of 1 min at 0.02 mW 488 nm-laser power at the output. Scale bar, 10 μm. (D) Line profiles of highlighted regions in (C) (dotted line, before activation; full line, after activation).

Journal: Frontiers in Cell and Developmental Biology

Article Title: Aberrant Phase Separation of FUS Leads to Lysosome Sequestering and Acidification

doi: 10.3389/fcell.2021.716919

Figure Lengend Snippet: Photoactivation of FUS condensation. (A) Scheme of full-length FUS protein (top) and chimeric constructs used in this study (bottom) (IDR, intrinsically disordered region; RRM and RGG, RNA-binding motives; Zn, Zn-finger). (B) Cartoon of the imaging assay in HEK cells. (C) Representative images (z-projections) before (left) and after (right) photoactivation of 1 min at 0.02 mW 488 nm-laser power at the output. Scale bar, 10 μm. (D) Line profiles of highlighted regions in (C) (dotted line, before activation; full line, after activation).

Article Snippet: The DNA fragment encoding the IDR of human FUS (residues 1-215, extracted from Addgene plasmid #60362) was inserted by PCR-based cloning into the pCry2-mCh vector using Xho I- Kpn I restriction sites ( ).

Techniques: Construct, RNA Binding Assay, Imaging, Activation Assay